68 Ga-labelled desferrioxamine-B for bacterial infection imaging

0540695 - MBÚ 2021 RIV DE eng J - Journal Article
Petřík, M. - Umlaufová, E. - Raclavský, V. - Palyzová, Andrea - Havlíček, Vladimír - Pfister, J. - Mair, C. - Nový, Z. - Popper, M. - Hajdúch, M. - Decristoforo, C.
68 Ga-labelled desferrioxamine-B for bacterial infection imaging.
European Journal of Nuclear Medicine and Molecular Imaging. Roč. 48, č. 2 (2021), s. 372-382. ISSN 1619-7070. E-ISSN 1619-7089
R&D Projects: GA MŠMT(CZ) LO1509; GA ČR(CZ) GA19-10907S
Institutional support: RVO:61388971
Keywords : Desferrioxamine-B * Gallium-68 * pet * Infection * Imaging
OECD category: Microbiology
Impact factor: 10.057, year: 2021
Method of publishing: Open access
https://link.springer.com/article/10.1007%2Fs00259-020-04948-y

Purpose With the increase of especially hospital-acquired infections, timely and accurate diagnosis of bacterial infections is crucial for effective patient care. Molecular imaging has the potential for specific and sensitive detection of infections. Siderophores are iron-specific chelators recognized by specific bacterial transporters, representing one of few fundamental differences between bacterial and mammalian cells. Replacing iron by gallium-68 without loss of bioactivity is possible allowing molecular imaging by positron emission tomography (PET). Here, we report on the preclinical evaluation of the clinically used siderophore, desferrioxamine-B (Desferal (R), DFO-B), radiolabelled with(68)Ga for imaging of bacterial infections. Methods In vitro characterization of [Ga-68]Ga-DFO-B included partition coefficient, protein binding and stability determination. Specific uptake of [Ga-68]Ga-DFO-B was tested in vitro in different microbial cultures. In vivo biodistribution was studied in healthy mice and dosimetric estimation for human setting performed. PET/CT imaging was carried out in animal infection models, representing the most common pathogens. Results DFO-B was labelled with(68)Ga with high radiochemical purity and displayed hydrophilic properties, low protein binding and high stability in human serum and PBS. The high in vitro uptake of [Ga-68]Ga-DFO-B in selected strains ofPseudomonas aeruginosa,Staphylococcus aureusandStreptococcus agalactiaecould be blocked with an excess of iron-DFO-B. [Ga-68]Ga-DFO-B showed rapid renal excretion and minimal retention in blood and other organs in healthy mice. Estimated human absorbed dose was 0.02 mSv/MBq. PET/CT images of animal infection models displayed high and specific accumulation of [Ga-68]Ga-DFO-B in bothP. aeruginosaandS. aureusinfections with excellent image contrast. No uptake was found in sterile inflammation, heat-inactivatedP. aeruginosaorS. aureusandEscherichia colilacking DFO-B transporters. Conclusion DFO-B can be easily radiolabelled with(68)Ga and displayed suitable in vitro characteristics and excellent pharmacokinetics in mice. The high and specific uptake of [Ga-68]Ga-DFO-B byP. aeruginosaandS. aureuswas confirmed both in vitro and in vivo, proving the potential of [Ga-68]Ga-DFO-B for specific imaging of bacterial infections. As DFO-B is used in clinic for many years and the estimated radiation dose is lower than for other(68)Ga-labelled radiopharmaceuticals, we believe that [Ga-68]Ga-DFO-B has a great potential for clinical translation.
Permanent Link: http://hdl.handle.net/11104/0318315