Catalase and Ascorbate Peroxidase in Euglenozoan Protists

0540625 - BC 2021 RIV CH eng J - Journal Article
Škodová-Sveráková, Ingrid - Záhonová, Kristýna - Bučková, B. - Füssy, Z. - Yurchenko, V. - Lukeš, Julius
Catalase and Ascorbate Peroxidase in Euglenozoan Protists.
Pathogens. Roč. 9, č. 4 (2020), č. článku 317. E-ISSN 2076-0817
R&D Projects: GA MŠMT(CZ) LL1601; GA ČR(CZ) GA18-15962S; GA ČR(CZ) GA20-07186S; GA MŠMT(CZ) EF16_019/0000759
Institutional support: RVO:60077344
Keywords : hydrogen-peroxide * glutathione-reductase * oxidative stress * leishmania-major * tobacco plants * gene family * metabolism * trypanothione * prediction * antioxidants * Euglenozoa * ascorbate peroxidase * catalase * enzymatic activity * phylogeny
OECD category: Microbiology
Impact factor: 3.492, year: 2020
Method of publishing: Open access
https://www.mdpi.com/2076-0817/9/4/317

In this work, we studied the biochemical properties and evolutionary histories of catalase (CAT) and ascorbate peroxidase (APX), two central enzymes of reactive oxygen species detoxification, across the highly diverse clade Eugenozoa. This clade encompasses free-living phototrophic and heterotrophic flagellates, as well as obligate parasites of insects, vertebrates, and plants. We present evidence of several independent acquisitions of CAT by horizontal gene transfers and evolutionary novelties associated with the APX presence. We posit that Euglenozoa recruit these detoxifying enzymes for specific molecular tasks, such as photosynthesis in euglenids and membrane-bound peroxidase activity in kinetoplastids and some diplonemids.
Permanent Link: http://hdl.handle.net/11104/0318246