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Efficient allele conversion in mouse zygotes and primary cells based on electroporation of Cre protein

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    0540328 - ÚMG 2023 RIV US eng J - Journal Article
    Jeníčková, Irena - Kašpárek, Petr - Petrezselyova, Silvia - Eliáš, Jan - Procházka, Jan - Kopkanová, Jana - Navrátil, Michal - Bařinka, Cyril - Sedláček, Radislav
    Efficient allele conversion in mouse zygotes and primary cells based on electroporation of Cre protein.
    Methods. Roč. 191, JUL (2021), s. 87-94. ISSN 1046-2023. E-ISSN 1095-9130
    R&D Projects: GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) LM2015040; GA MŠMT(CZ) LM2018126; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT ED2.1.00/19.0395
    Institutional support: RVO:68378050 ; RVO:86652036
    Keywords : Conditional * Delivery * EC * MEF * Recombination * Transgenesis * loxP
    OECD category: Biochemistry and molecular biology; Biochemistry and molecular biology (BTO-N)
    Impact factor: 4.647, year: 2021
    Method of publishing: Open access
    https://www.sciencedirect.com/science/article/abs/pii/S1046202320301146?via%3Dihub

    Cre-loxP recombination system is a powerful tool for genome engineering. One of its applications is found in genetic mouse models that often require to induce Cre recombination in preimplantation embryos. Here, we describe a technically simple, affordable and highly efficient protocol for Cre protein delivery into mouse zygotes by electroporation. We show that electroporation based delivery of Cre has no negative impact on embryo survival and the method can be easily combined with in vitro fertilization resulting in a significantly faster generation of desired models. Lastly, we demonstrate that Cre protein electroporation is suitable for allelic conversion in primary cells derived from conditional mouse models.
    Permanent Link: http://hdl.handle.net/11104/0317973

     
     
Number of the records: 1  

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