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Basophils and Mast Cells. Methods and Protocols
- 1.0540018 - ÚMG 2021 RIV US eng M - Monography Chapter
Bambousková, Monika - Rubíková, Zuzana - Dráberová, Lubica - Dráber, Pavel - Dráber, Petr
Mast Cell Migration and Chemotaxis Assayed by Microscopy.
Basophils and Mast Cells. Methods and Protocols. New York: Humana Press, 2020, s. 293-310. ISBN 978-1-0716-0696-4
R&D Projects: GA ČR(CZ) GA17-20255S; GA ČR(CZ) GA17-20915S; GA ČR GA18-18521S; GA ČR(CZ) GA19-20716S; GA MŠMT(CZ) LTAUSA17052
Institutional support: RVO:68378050
Keywords : mast cell * chemotaxis * microscopy
OECD category: Immunology
https://link-springer-com.d360prx.biomed.cas.cz/protocol/10.1007%2F978-1-0716-0696-4_24
A better understanding of the molecular mechanisms leading to mast cell migration and chemotaxis is the long-term goal in mast cell research and is essential for comprehension of mast cell function in health and disease. Various techniques have been developed in recent decades for in vitro and in vivo assessment of mast cell motility and chemotaxis. In this chapter, three microscopy assays facilitating real-time quantification of mast cell chemotaxis and migration are described, focusing on individual cell tracking and data analysis.
Permanent Link: http://hdl.handle.net/11104/0317696
Number of the records: 1