Cytokinin-regulated expression of arabidopsis thaliana pap genes and its implication for the expression of chloroplast-encoded genes

0539951 - ÚEB 2021 RIV CH eng J - Journal Article
Andreeva, A. A. - Vaňková, Radomíra - Bychkov, I. A. - Kudryakova, N.V. - Danilova, M. N. - Lacek, Jozef - Pojidaeva, E. S. - Kusnetsov, V.
Cytokinin-regulated expression of arabidopsis thaliana pap genes and its implication for the expression of chloroplast-encoded genes.
Biomolecules. Roč. 10, č. 12 (2020), s. 1-18, č. článku 1658. E-ISSN 2218-273X
Institutional support: RVO:61389030
Keywords : Arabidopsis thaliana * Chloroplast biogenesis * Cytokinin * PEP-associated proteins * Plastid transcription machinery
OECD category: Biochemistry and molecular biology
Impact factor: 4.879, year: 2020
Method of publishing: Open access
http://doi.org/10.3390/biom10121658

Cytokinins (CKs) are known to regulate the biogenesis of chloroplasts under changing environmental conditions and at different stages of plant ontogenesis. However, the underlying mechanisms are still poorly understood. Apparently, the mechanisms can be duplicated in several ways, including the influence of nuclear genes that determine the expression of plastome through the two-component CK regulatory circuit. In this study, we evaluated the role of cytokinins and CK signaling pathway on the expression of nuclear genes for plastid RNA polymerase-associated proteins (PAPs). Cytokinin induced the expression of all twelve Arabidopsis thaliana PAP genes irrespective of their functions via canonical CK signaling pathway but this regulation might be indirect taking into consideration their different functions and versatile structure of promoter regions. The disruption of PAP genes contributed to the abolishment of positive CK effect on the accumulation of the chloroplast gene transcripts and transcripts of the nuclear genes for plastid transcription machinery as can be judged from the analysis of pap1 and pap6 mutants. However, the CK regulatory circuit in the mutants remained practically unperturbed. Knock-out of PAP genes resulted in cytokinin overproduction as a consequence of the strong up-regulation of the genes for CK synthesis.
Permanent Link: http://hdl.handle.net/11104/0317638