Differentially Expressed Genes Shared by Two Distinct Cytoplasmic Male Sterility (CMS) Types of Silene vulgaris Suggest the Importance of Oxidative Stress in Pollen Abortion

0539943 - ÚEB 2021 RIV CH eng J - Journal Article
Krüger, Manuela - Abeyawardana, Oushadee A.J. - Krüger, Claudia - Juříček, Miloslav - Štorchová, Helena
Differentially Expressed Genes Shared by Two Distinct Cytoplasmic Male Sterility (CMS) Types of Silene vulgaris Suggest the Importance of Oxidative Stress in Pollen Abortion.
Cells. Roč. 9, č. 12 (2020), č. článku 2700. E-ISSN 2073-4409
R&D Projects: GA ČR GA16-09220S; GA MŠMT(CZ) EF16_019/0000738
Institutional support: RVO:61389030
Keywords : cytoplasmic male sterility * differential gene expression * Silene vulgaris
OECD category: Biochemical research methods
Impact factor: 6.600, year: 2020
Method of publishing: Open access
http://doi.org/10.3390/cells9122700

Cytoplasmic male sterility (CMS), encoded by the interacting mitochondrial and nuclear genes, causes pollen abortion or non-viability. CMS is widely used in agriculture and extensively studied in crops. Much less is known about CMS in wild species. We performed a comparative transcriptomic analysis of male sterile and fertile individuals of Silene vulgaris, a model plant for the study of gynodioecy, to reveal the genes responsible for pollen abortion in this species. We used RNA-seq datasets previously employed for the analysis of mitochondrial and plastid transcriptomes of female and hermaphrodite flower buds, making it possible to compare the transcriptomes derived from three genomes in the same RNA specimen. We assembled de novo transcriptomes for two haplotypes of S. vulgaris and identified differentially expressed genes between the females and hermaphrodites, associated with stress response or pollen development. The gene for alternative oxidase was downregulated in females. The genetic pathways controlling CMS in S. vulgaris are similar to those in crops. The high number of the differentially expressed nuclear genes contrasts with the uniformity of organellar transcriptomes across genders, which suggests these pathways are evolutionarily conserved and that selective mechanisms may shield organellar transcription against changes in the cytoplasmic transcriptome.
Permanent Link: http://hdl.handle.net/11104/0317629