Testing of fibrous membranes with different scaffold morphology for development of 3D skin model

0539919 - ÚEM 2021 RIV SE eng C - Conference Paper (international conference)
Sovková, Věra - Vocetková, Karolína - Rampichová, Michala - Filová, Eva
Testing of fibrous membranes with different scaffold morphology for development of 3D skin model.
Key Engineering Materials. Bäch: Scientific, 2020, s. 148-154. ISBN 978-303571519-4.
[4th International Conference on Smart Materials Technologies. Salt Lake City (US), 02.08.2019-05.08.2019]
R&D Projects: GA MŠMT(CZ) LO1508; GA MPO(CZ) FV30086
Institutional support: RVO:68378041
Keywords : fibroblasts * keratinocytes * scaffold
OECD category: Biomaterials (as related to medical implants, devices, sensors)
https://www.scientific.net/KEM.834.148

Replacement of in vivo testing using advanced 3D constructs is an important challenge in tissue engineering applications. The cell culture material should be biocompatible and should mimic the natural microenvironment of the existing tissue. Nanofibrous scaffolds prepared by electrospinning from biocompatible polymers have suitable properties for cell culture in a 3D environment. Thanks to the high volume-to-surface ratio, controlled porous structure with high pore interconnection and microarchitecture in the nanoscale range, nanofibers are in the foreground of interest. We tested membranes with different topography with keratinocyte and fibroblast cell lines. Fibroblast showed stable growth with no difference among the scaffolds. On the other hand, keratinocytes preferred scaffolds with nanofiber morphology. © 2020 Trans Tech Publications Ltd, Switzerland.
Permanent Link: http://hdl.handle.net/11104/0318705