Generation and characterization of Aldh3-Cre transgenic mice as a tool for conditional gene deletion in postnatal cornea

0539745 - ÚMG 2021 RIV GB eng J - Journal Article
Sunny, Sweetu Susan - Láchová, Jitka - Dupačová, Naoko - Zitová, Anna - Kozmik, Zbyněk
Generation and characterization of Aldh3-Cre transgenic mice as a tool for conditional gene deletion in postnatal cornea.
Scientific Reports. Roč. 10, č. 1 (2020), č. článku 9083. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA ČR(CZ) GA18-20759S; GA MŠMT ED2.1.00/19.0395; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) LM2018126
Institutional support: RVO:68378050
Keywords : aldehyde dehydrogenases * expression * epithelium * cells * differentiation * inactivation * maintenance * movement * mutation
OECD category: Developmental biology
Impact factor: 4.380, year: 2020
Method of publishing: Open access
https://www.nature.com/articles/s41598-020-65878-1

Conditional gene targeting in mice by means of Cre-loxP strategy represents a powerful approach to study mammalian gene function. This approach is however dependent on the availability of suitable strains of mice with a tissue or time restricted activity of the Cre recombinase. Here we describe Aldh3-Cre transgenic mice as a useful tool to conditionally delete genes in cornea, a specialized transparent tissue found on the anterior-most part of the eye, which acts as a protective barrier and contributes to the refractive power. Using a set of floxed alleles we demonstrate high Aldh3-Cre activity in corneal epithelial cells, corneal stroma and conjunctival epithelial cells at postnatal stages. Aldh3-Cre will thus be particularly beneficial for functional analysis of genes which are vital for postnatal development of cornea and conjunctiva.
Permanent Link: http://hdl.handle.net/11104/0317444