A freshwater radiation of diplonemids

0539257 - BC 2021 RIV GB eng J - Journal Article
Mukherjee, Indranil - Salcher, Michaela M. - Andrei, Adrian-Stefan - Kavagutti, Vinicius Silva - Shabarova, Tatiana - Grujcic, V. - Haber, Markus - Layoun, Paul - Hodoki, Y. - Nakano, S. - Šimek, Karel - Ghai, Rohit
A freshwater radiation of diplonemids.
Environmental Microbiology. Roč. 22, č. 11 (2020), s. 4658-4668. ISSN 1462-2912. E-ISSN 1462-2920
R&D Projects: GA ČR(CZ) GX20-12496X; GA ČR(CZ) GA17-04828S; GA ČR(CZ) GA19-23469S
Grant - others:AV ČR(CZ) MSM200961801
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Institutional support: RVO:60077344
Keywords : multiple sequence alignment * microbial eukaryotes * marine * community * diversity
OECD category: Microbiology
Impact factor: 5.491, year: 2020
Method of publishing: Open access
https://doi.org/10.1111/1462-2920.15209

Diplonemids are considered marine protists and have been reported among the most abundant and diverse eukaryotes in the world oceans. Recently we detected the presence of freshwater diplonemids in Japanese deep freshwater lakes. However, their distribution and abundances in freshwater ecosystems remain unknown. We assessed abundance and diversity of diplonemids from several geographically distant deep freshwater lakes of the world by amplicon-sequencing, shotgun metagenomics and catalysed reporter deposition-fluorescentin situhybridization (CARD-FISH). We found diplonemids in all the studied lakes, albeit with low abundances and diversity. We assembled long 18S rRNA sequences from freshwater diplonemids and showed that they form a new lineage distinct from the diverse marine clades. Freshwater diplonemids are a sister-group to a marine clade, which are mainly isolates from coastal and bay areas, suggesting a recent habitat transition from marine to freshwater habitats. Images of CARD-FISH targeted freshwater diplonemids suggest they feed on bacteria. Our analyses of 18S rRNA sequences retrieved from single-cell genomes of marine diplonemids show they encode multiple rRNA copies that may be very divergent from each other, suggesting that marine diplonemid abundance and diversity both have been overestimated. These results have wider implications on assessing eukaryotic abundances in natural habitats by using amplicon-sequencing alone.
Permanent Link: http://hdl.handle.net/11104/0316927