In Vitro Comparison of Passive and Active Clinical Proton Beams

0539168 - ÚMG 2021 RIV CH eng J - Journal Article
Michaelidesova, A. - Vachelova, J. - Klementová, Jana - Urban, T. - Brabcova, K. P. - Kaczor, S. - Falk, M. - Falková, I. - Depes, D. - Vondráček, V. - Davídková, M.
In Vitro Comparison of Passive and Active Clinical Proton Beams.
International Journal of Molecular Sciences. Roč. 21, č. 16 (2020), č. článku 5650. E-ISSN 1422-0067
R&D Projects: GA MŠMT LM2015063
Institutional support: RVO:68378050
Keywords : proton therapy * pencil beam scanning * double scattering * cell survival
OECD category: Cell biology
Impact factor: 5.924, year: 2020
Method of publishing: Open access
https://www.mdpi.com/1422-0067/21/16/5650

Nowadays, the irradiation methodology in proton therapy is switching from the use of passively scattered beams to active pencil beams due to the possibility of more conformal dose distributions. The dose rates of active pencil beams are much higher than those of passive beams. The purpose of this study was to investigate whether there is any difference in the biological effectiveness of these passive and active irradiation modes. The beam qualities of double scattering and pencil beam scanning were measured dosimetrically and simulated using the Monte Carlo code. Using the medulloblastoma cell line DAOY, we performed an in vitro comparison of the two modes in two positions along the dose-deposition curve plateau and inside the Bragg peak. We followed the clonogenic cell survival, apoptosis, micronuclei, and gamma H2AX assays as biological endpoints. The Monte Carlo simulations did not reveal any difference between the beam qualities of the two modes. Furthermore, we did not observe any statistically significant difference between the two modes in the in vitro comparison of any of the examined biological endpoints. Our results do not show any biologically relevant differences related to the different dose rates of passive and active proton beams.
Permanent Link: http://hdl.handle.net/11104/0316899