Oxidative Damage in Sporadic Colorectal Cancer: Molecular Mapping of Base Excision Repair Glycosylases in Colorectal Cancer Patients

0538754 - ÚEM 2022 RIV CH eng J - Journal Article
Vodička, Pavel - Urbanová, M. - Makovický, P. - Tomášová, Kristýna - Kroupa, Michal - Štětina, R. - Opattová, Alena - Kostovčíková, Klára - Šišková, Anna - Schneiderová, M. - Vymetálková, Veronika - Vodičková, Ludmila
Oxidative Damage in Sporadic Colorectal Cancer: Molecular Mapping of Base Excision Repair Glycosylases in Colorectal Cancer Patients.
International Journal of Molecular Sciences. Roč. 21, č. 7 (2020), č. článku 2473. E-ISSN 1422-0067
R&D Projects: GA ČR(CZ) GA18-09709S; GA ČR(CZ) GA19-10543S; GA MZd(CZ) NV15-27580A; GA MZd(CZ) NV18-03-00199
Institutional support: RVO:68378041 ; RVO:61388971
Keywords : oxidative DNA damage * DNA repair * base excision repair (BER)glycosylases
OECD category: Genetics and heredity (medical genetics to be 3); Microbiology (MBU-M)
Impact factor: 5.924, year: 2020
Method of publishing: Open access
https://www.mdpi.com/1422-0067/21/7/2473

Oxidative stress with subsequent premutagenic oxidative DNA damage has been implicated in colorectal carcinogenesis. The repair of oxidative DNA damage is initiated by lesion-specific DNA glycosylases (hOGG1, NTH1, MUTYH). The direct evidence of the role of oxidative DNA damage and its repair is proven by hereditary syndromes (MUTYH-associated polyposis, NTHL1-associated tumor syndrome), where germline mutations cause loss-of-function in glycosylases of base excision repair, thus enabling the accumulation of oxidative DNA damage and leading to the adenoma-colorectal cancer transition. Unrepaired oxidative DNA damage often results in G:C>T:A mutations in tumor suppressor genes and proto-oncogenes and widespread occurrence of chromosomal copy-neutral loss of heterozygosity. However, the situation is more complicated in complex and heterogeneous disease, such as sporadic colorectal cancer. Here we summarized our current knowledge of the role of oxidative DNA damage and its repair on the onset, prognosis and treatment of sporadic colorectal cancer. Molecular and histological tumor heterogeneity was considered. Our study has also suggested an additional important source of oxidative DNA damage due to intestinal dysbiosis. The roles of base excision repair glycosylases (hOGG1, MUTYH) in tumor and adjacent mucosa tissues of colorectal cancer patients, particularly in the interplay with other factors (especially microenvironment), deserve further attention. Base excision repair characteristics determined in colorectal cancer tissues reflect, rather, a disease prognosis. Finally, we discuss the role of DNA repair in the treatment of colon cancer, since acquired or inherited defects in DNA repair pathways can be effectively used in therapy.
Permanent Link: http://hdl.handle.net/11104/0316488