Separation based characterization methods for the N-glycosylation analysis of prostate-specific antigen

0538553 - ÚIACH 2022 RIV NL eng J - Journal Article
Reider, B. - Jarvas, G. - Křenková, Jana - Guttman, A.
Separation based characterization methods for the N-glycosylation analysis of prostate-specific antigen.
Journal of Pharmaceutical and Biomedical Analysis. Roč. 194, FEB (2021), č. článku 113797. ISSN 0731-7085. E-ISSN 1873-264X
R&D Projects: GA ČR(CZ) GA18-00062S
Grant - others:AV ČR(CZ) MTA-19-06
Program: Bilaterální spolupráce
Institutional support: RVO:68081715
Keywords : biomarkers * glycans * glycosylation * prostate cancer
OECD category: Analytical chemistry
Impact factor: 3.571, year: 2021
Method of publishing: Limited access

Prostate cancer has the highest malignancy rate diagnosed in men worldwide. Albeit, the gold standard serum prostate-specific antigen (PSA) assays reduced the mortality rate of the disease, the number of false positive diagnoses steeply increased. Therefore, there is an urgent need for complementary biomarkers to enhance the specificity and selectivity of current diagnostic methods. Information about PSA glycosylation can help to fulfill this gap as alterations of its carbohydrate moieties due to cancerous transformation may represent additional markers to distinguish malignant from benign tumors. However, development of suitable methods and instrumentations to investigate the N-glycosylation profile of PSA represents a challenge. In this paper, we critically review the current bioanalytical trends and strategies in the field of PSA glycobiomarker research focusing on separation based characterization methods.
Permanent Link: http://hdl.handle.net/11104/0316343