Proteomic Analysis of Whole Saliva in Relation to Dental Caries Resistance

0538460 - FGÚ 2021 RIV CZ eng J - Journal Article
Kulhavá, Lucie - Eckhardt, Adam - Pataridis, Statis - Foltán, R. - Mikšík, Ivan
Proteomic Analysis of Whole Saliva in Relation to Dental Caries Resistance.
Folia Biologica. Roč. 66, č. 2 (2020), s. 72-80. ISSN 0015-5500. E-ISSN 0015-5500
R&D Projects: GA MZd(CZ) NV17-31564A
Institutional support: RVO:67985823
Keywords : dental caries * fractions of whole human saliva * label-free quantification * mass spectrometry * proteins
OECD category: Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions (pharmacogenomics, gene-based therapeutics)
Impact factor: 0.906, year: 2020
Method of publishing: Open access
https://fb.cuni.cz/file/5923/fb2020a0009.pdf

Saliva contains possible biomarkers that are associated with dental caries. The present study aimed to analyse differences in the abundance of proteins in the saliva between caries-positive (CP, N = 15) and caries-free (CF, N = 12) males and to compare differences in the abundance of proteins between two saliva sample fractions (supernatant and pellet). We found 14 differently significantly expressed proteins in the CF group when comparing the supernatant fractions of the CP and CF groups, and three proteins in the pellet fractions had significantly higher expression in the CP group. Our results indicate very specific protein compositions of the saliva in relation to dental caries resistance (the saliva of the CP group mainly contained pellet proteins and the saliva of the CF group mainly contained supernatant proteins). This was the first time that the saliva pellet fraction was analysed in relation to the dental caries status. We detected specific calcium-binding proteins that could have decalcified enamel in the saliva pellet of the CP group. We also observed significantly up-regulated immune proteins in the saliva supernatant of the CF group that could play an important role in the caries prevention. The particular protein compositions of the saliva pellet and supernatant in the groups with different susceptibilities to tooth decay is a promising finding for future research.
Permanent Link: http://hdl.handle.net/11104/0316251