Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 tat on hydrogen-terminated surface

0538344 - FZÚ 2021 RIV MY eng J - Journal Article
Ahmad, N.A. - Rahim, R.A. - Rezek, Bohuslav - Kromka, Alexander - Ismail, N.S. - Gopinath, S.Ch.B. - Ižák, Tibor - Procházka, V. - Faudzi, F.N.M. - Abidin, A.S.Z. - Maidizn, N.N.M.
Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 tat on hydrogen-terminated surface.
International Journal of Nanoelectronics and Materials. Roč. 13, č. 2 (2020), s. 295-306. ISSN 1985-5761
R&D Projects: GA MŠMT(CZ) EF16_019/0000760
Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Institutional support: RVO:68378271
Keywords : aptamer * electrolyte-gated field effect transistor * HIV-1 Tat * nanocrystalline diamonds
OECD category: Nano-materials (production and properties)
Method of publishing: Open access
https://ijneam.unimap.edu.my/images/PDF/IJNEAM%20APR%202020/Vol_13_No_2_2020_7_295-306.pdf

This research investigated on Trans-activator of transcription (Tat) protein, a powerful viral gene activator that plays a pivotal role in the primary stage of the human immunodeficiency virus type 1 (HIV-1) replication. Dose-dependent interactions of HIV-1 Tat on NCD-EGFET-based RNA aptamer sensing surface were monitored and attained the detection down to 10 fM. The linear regression curve with 3σ estimation professed the sensitivity range to be 31.213 mV/log10 [Tat Concentration]M and the limit of detection of 6.18 fM. The selectivity analysis of NCD-EGFET was conducted with different proteins from HIV (Nef and p24) and Bovine Serum Albumin. Furthermore, to practice in the clinical application, HIV-1 Tat was spiked into the human blood serum and it displayed the genuine non-fouling interaction with the aptamer. The attained high-performance signal enhancement with nanocrystalline diamond-biosensing aids to circumvent the issues in the current diagnosis.
Permanent Link: http://hdl.handle.net/11104/0316158