Comprehensive proteomic investigation of infectious and inflammatory changes in late preterm prelabour rupture of membranes

0538100 - FGÚ 2021 RIV GB eng J - Journal Article
Vajrychová, M. - Stráník, J. - Pimková, K. - Barman, M. - Kukla, R. - Zedníková, Petra - Bolehovská, R. - Plíšková, L. - Hornychová, H. - Andrýs, C. - Tambor, V. - Lenčo, J. - Jacobsson, B. - Kacerovský, M.
Comprehensive proteomic investigation of infectious and inflammatory changes in late preterm prelabour rupture of membranes.
Scientific Reports. Roč. 10, č. 1 (2020), č. článku 17696. ISSN 2045-2322. E-ISSN 2045-2322
Institutional support: RVO:67985823
Keywords : proteomics * proteins * premature rupture
OECD category: Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions (pharmacogenomics, gene-based therapeutics)
Impact factor: 4.380, year: 2020
Method of publishing: Open access
https://www.nature.com/articles/s41598-020-74756-9

Preterm prelabour rupture of membranes beyond the 34th week of gestation (late PPROM) is frequently associated with the risk of the microbial invasion of the amniotic fluid (MIAC) and histological chorioamnionitis (HCA). Hence, we employed a Tandem Mass Tag-based approach to uncover amniotic fluid proteome response to the presence of MIAC and HCA in late PPROM. Protein dysregulation was associated with only five cases in the group of 15 women with confirmed MIAC and HCA. Altogether, 138 amniotic fluid proteins were changed in these five cases exclusively. These proteins were particularly associated with excessive neutrophil responses to infection, such as neutrophil degranulation and extracellular trap formation. We believe that the quantification of these proteins in amniotic fluid may assist in revealing women with the highest risk of excessive inflammatory response in late PPROM.
Permanent Link: http://hdl.handle.net/11104/0315915