Arbuscular Mycorrhizal Fungi. Methods and Protocols

0538085 - BÚ 2021 RIV US eng M - Monography Chapter
Janoušková, Martina - Caklová, Petra
Molecular quantification of arbuscular mycorrhizal fungal root colonization.
Arbuscular Mycorrhizal Fungi. Methods and Protocols. New York: Springer Science + Business Media, 2020 - (Ferrol, N.; Lanfranco, L.), s. 73-91. Methods in Molecular Biology, 2146. ISBN 978-1-0716-0602-5
Institutional support: RVO:67985939
Keywords : arbuscular mycorrhiza * quantitative real-time PCR * root colonization
OECD category: Mycology
https://doi.org/10.1007/978-1-0716-0603-2

Specific quantification of root-colonizing arbusular mycorrhizal fungi (AMF) by quantitative real-time PCR is a high-throughput technique, most suitable for determining abundances of AMF species or isolates in previously characterized experimental systems. The use of a suitable assay is crucial for a correct data collection but also highly specific for each experimental system and is therefore covered by general recommendations. Subsequently, specific steps are described for the validation of the assay using a standard dilution series, the determination of appropriate dilution sof DNA extrracts from roots, and the quantification of the gene copy numbers in samples including calculations.
Permanent Link: http://hdl.handle.net/11104/0315904