Colonization of Germ-Free Piglets with Mucinolytic and Non-Mucinolytic Bifidobacterium boum Strains Isolated from the Intestine of Wild Boar and Their Interference with Salmonella Typhimurium

0538008 - MBÚ 2021 RIV CH eng J - Journal Article
Šplíchalová, Alla - Pechar, R. - Killer, Jiří - Šplíchalová, Zdislava - Neužil Bunešová, V. - Vlková, E. - Šubrtová Salmonová, H. - Šplíchal, Igor
Colonization of Germ-Free Piglets with Mucinolytic and Non-Mucinolytic Bifidobacterium boum Strains Isolated from the Intestine of Wild Boar and Their Interference with Salmonella Typhimurium.
Microorganisms. Roč. 8, č. 12 (2020), č. článku 2002. E-ISSN 2076-2607
R&D Projects: GA ČR GA13-08803S
Institutional support: RVO:61388971 ; RVO:67985904
Keywords : Bifidobacterium boum * Salmonella Typhimurium * goblet cells * mucin * mucinolytic * germ-free
OECD category: Microbiology; Microbiology (UZFG-Y)
Impact factor: 4.128, year: 2020
Method of publishing: Open access
https://www.mdpi.com/2076-2607/8/12/2002

Non-typhoidal Salmonella serovars are worldwide spread foodborne pathogens that cause diarrhea in humans and animals. Colonization of gnotobiotic piglet intestine with porcine indigenous mucinolytic Bifidobacterium boum RP36 strain and non-mucinolytic strain RP37 and their interference with Salmonella Typhimurium infection were compared. Bacterial interferences and impact on the host were evaluated by clinical signs of salmonellosis, bacterial translocation, goblet cell count, mRNA expression of mucin 2, villin, claudin-1, claudin-2, and occludin in the ileum and colon, and plasmatic levels of inflammatory cytokines IL-8, TNF-alpha, and IL-10. Both bifidobacterial strains colonized the intestine comparably. Neither RP36 nor RP37 B. boum strains effectively suppressed signs of salmonellosis. Both B. boum strains suppressed the growth of S. Typhimurium in the ileum and colon. The mucinolytic RP36 strain increased the translocation of S. Typhimurium into the blood, liver, and spleen.
Permanent Link: http://hdl.handle.net/11104/0315844