Inhibition of miR-152 during In Vitro Maturation Enhances the Developmental Potential of Porcine Embryos

0537771 - ÚŽFG 2021 RIV CH eng J - Journal Article
Gad, Ahmed - Murín, Matěj - Němcová, Lucie - Bártková, Alexandra - Laurinčík, Jozef - Procházka, Radek
Inhibition of miR-152 during In Vitro Maturation Enhances the Developmental Potential of Porcine Embryos.
Animals. Roč. 10, č. 12 (2020), č. článku 2289. ISSN 2076-2615. E-ISSN 2076-2615
R&D Projects: GA MŠMT EF15_003/0000460
Institutional support: RVO:67985904
Keywords : miR-152 * porcine * oocyte * blastocyst rate
OECD category: Developmental biology
Impact factor: 2.752, year: 2020
Method of publishing: Open access
https://www.mdpi.com/2076-2615/10/12/2289

MiR-152 is a highly conserved miRNA across different species and plays a role in the regulation of cell differentiation, proliferation, and survival. However, the exact role of miR-152 in oocyte and embryo development is not yet known. In this study, we specifically manipulated the expression level of miR-152 in porcine cumulus-oocyte complexes (COCs) and monitored their developmental competence until the blastocyst stage. We mainly found that a suppressed expression of miR-152 during oocyte maturation significantly improved the blastocyst rate. Our results indicate that this negative correlation between miR-152 during oocyte maturation and the blastocyst rate in pigs could be through targeting IGF system components during oocyte development. These results provide more insights into the role of miRNAs during oocyte and embryonic development that could improve the in vitro production system for mammalian embryos.
Permanent Link: http://hdl.handle.net/11104/0315611