Analysis of hyaluronan and its derivatives using chromatographic and mass spectrometric techniques

0537559 - MBÚ 2021 RIV GB eng J - Journal Article
Šimek, M. - Lemr, Karel - Hermanová, M. - Havlíček, Vladimír
Analysis of hyaluronan and its derivatives using chromatographic and mass spectrometric techniques.
Carbohydrate Polymers. Roč. 250, DEC15 (2020), č. článku 117014. ISSN 0144-8617. E-ISSN 1879-1344
Institutional support: RVO:61388971
Keywords : Hyaluronic acid * Oligosaccharides * Polysaccharide derivative
OECD category: Microbiology
Impact factor: 9.381, year: 2020
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0144861720311875

The aim of this paper is to review chromatographic and mass-spectrometric methods and underline the best analytical approaches for successful analysis of various hyaluronic acid species in different types of samples. Hyaluronan-degrading enzymes and chemical depolymerization produce dior oligosaccharides suitable for hyaluronan quantification or structural characterization of hyaluronan derivatives. Efficient purification and pre-column derivatization of hyaluronan disaccharides by reductive amination allow subnanogram quantification in biological samples. The chromatographic separation is capable to distinguish all glycosaminoglycans disaccharides and to resolve hyaluronan fragments with 2-40 monomers. Using electrospray ionization or matrix assisted laser desorption ionization, hyaluronan fragments up to 8 kDa or 41 kDa, respectively, can be observed. One- or two-dimensional chromatographic separation with higly sensitive mass-spectrometric detection is an indispensable tool for revealing substituent position, extent of modification and substitution patterns of chemically modified hyaluronan derivatives. It is essential for studying structure-biological function relationships of hyaluronan and its derivatives.
Permanent Link: http://hdl.handle.net/11104/0315379