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Histone post-translational modifications in Silene latifolia X and Y chromosomes suggest a mammal-like dosage compensation system

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    0537554 - BFÚ 2021 RIV IE eng J - Journal Article
    Rodriguez Lorenzo, José Luis - Hubinský, Marcel - Vyskot, Boris - Hobza, Roman
    Histone post-translational modifications in Silene latifolia X and Y chromosomes suggest a mammal-like dosage compensation system.
    Plant Science. Roč. 299, č. 2020 (2020), č. článku 110528. ISSN 0168-9452. E-ISSN 1873-2259
    R&D Projects: GA ČR(CZ) GA18-06147S
    Institutional support: RVO:68081707
    Keywords : sex-chromosomes * dna methylation * dioecious plant * recombination suppression * repetitive sequences * evolution * degeneration
    OECD category: Biochemistry and molecular biology
    Impact factor: 4.729, year: 2020
    Method of publishing: Limited access
    https://www.sciencedirect.com/science/article/pii/S0168945220301333?via%3Dihub

    Silene latifolia is a model organism to study evolutionary young heteromorphic sex chromosome evolution in plants. Previous research indicates a Y-allele gene degeneration and a dosage compensation system already operating. Here, we propose an epigenetic approach based on analysis of several histone post-translational modifications (PTMs) to find the first epigenetic hints of the X:Y sex chromosome system regulation in S. latifolia. Through chromatin immunoprecipitation we interrogated six genes from X and Y alleles. Several histone PTMS linked to DNA methylation and transcriptional repression (H3K27me3, H3K23me, H3K9me2 and H3K9me3) and to transcriptional activation (H3K4me3 and H4K5, 8, 12, 16ac) were used. DNA enrichment (Immunoprecipitated DNA/input DNA) was analyzed and showed three main results: (i) promoters of the Y allele are associated with heterochromatin marks, (ii) promoters of the X allele in males are associated with activation of transcription marks and finally, (iii) promoters of X alleles in females are associated with active and repressive marks. Our finding indicates a transcription activation of X allele and transcription repression of Y allele in males. In females we found a possible differential regulation (up X-1, down X-2) of each female X allele. These results agree with the mammal-like epigenetic dosage compensation regulation.
    Permanent Link: http://hdl.handle.net/11104/0315375

     
     
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