Hepatic tumor cell morphology plasticity under physical constraints in 3D cultures driven by YAP-mTOR axis

0537345 - FZÚ 2021 RIV CH eng J - Journal Article
Frtús, Adam - Smolková, Barbora - Uzhytchak, Mariia - Lunova, Mariia - Jirsa, M. - Hof, Martin - Jurkiewicz, Piotr - Lozinsky, V. - Wolfová, L. - Petrenko, Y. - Kubinová, Šárka - Dejneka, Alexandr - Lunov, Oleg
Hepatic tumor cell morphology plasticity under physical constraints in 3D cultures driven by YAP-mTOR axis.
Pharmaceuticals. Roč. 13, č. 12 (2020), s. 1-25, č. článku 430. E-ISSN 1424-8247
R&D Projects: GA MŠMT LTC19040
Institutional support: RVO:68378271 ; RVO:61388955
Keywords : mechanotransduction * YAP * mTOR * autophagy * 3D cultures * cytoskeleton * cell plasticity
OECD category: Biophysics; Physical chemistry (UFCH-W)
Impact factor: 5.863, year: 2020
Method of publishing: Open access

Recent studies undoubtedly show that the mammalian target of rapamycin (mTOR) and the Hippo–Yes-associated protein 1 (YAP) pathways are important mediators of mechanical cues. The crosstalk between these pathways as well as de-regulation of their signaling has been implicated in multiple tumor types, including liver tumors. Additionally, physical cues from 3D microenvironments have been identified to alter gene expression and di erentiation of di erent cell lineages. However, it remains incompletely understood how physical constraints originated in 3D cultures a ect cell plasticity and what the key mediators are of such process. In this work, we use collagen sca olds as a model of a soft 3D microenvironment to alter cellular size and study the mechanotransduction that regulates that process.
Permanent Link: http://hdl.handle.net/11104/0315072