Genetic tool development in marine protists: emerging model organisms for experimental cell biology

0537241 - BC 2021 RIV GB eng J - Journal Article
Faktorová, Drahomíra - Nisbet, R.E.R. - Robledo, J.A.F. - Casacuberta, E. - Sudek, L. - Allen, A. E. - Ares, M. - Areste, C. - Balestreri, C. - Barbrook, A.C. - Beardslee, P. - Bender, S. - Booth, D.S. - Bowler, C. - Breglia, S.A. - Brownlee, C. - Burger, G. - Cerutti, H. - Cesaroni, R. - Chiurillo, M.A. - Clemente, T. - Coles, D.B. - Collier, J.L. - Cooney, E.C. - Coyne, K. - Docampo, R. - Dupont, C.L. - Edgcomb, V. - Einarsson, E. - Elustondo, P.A. - Federici, F. - Freire-Beneitez, V. - Freyria, N.J. - Fukuda, K. - Garcia, P.A. - Girguis, P.R. - Gomaa, F. - Hampl, V. - Kachale, Ambar - Kaur, Binnypreet - Keeling, P. J. - Vanclová-Novák, A.M.G. - Pyrih, J. - Lukeš, Julius … Total 113 authors
Genetic tool development in marine protists: emerging model organisms for experimental cell biology.
Nature Methods. Roč. 17, č. 5 (2020), s. 481-494. ISSN 1548-7091. E-ISSN 1548-7105
R&D Projects: GA MŠMT(CZ) EF16_019/0000759
Grant - others:Gordon and Betty Moore Foundation(US) GMBM4982.01
Institutional support: RVO:60077344
Keywords : homologous recombination * transformation * genome
OECD category: Microbiology
Impact factor: 28.547, year: 2020
Method of publishing: Open access
https://www.nature.com/articles/s41592-020-0796-x

Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.
Permanent Link: http://hdl.handle.net/11104/0314982