Directionality of light absorption and emission in representative fluorescent proteins

0537175 - ÚOCHB 2021 RIV US eng J - Journal Article
Myšková, Jitka - Rybakova, Olga - Brynda, Jiří - Khoroshyy, Petro - Bondar, Alexey - Lazar, Josef
Directionality of light absorption and emission in representative fluorescent proteins.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 117, č. 51 (2020), s. 32395-32401. ISSN 0027-8424. E-ISSN 1091-6490
R&D Projects: GA MŠMT(CZ) EF16_019/0000729
Institutional support: RVO:61388963
Keywords : fluorescent protein * transition dipole moment * polarization microscopy
OECD category: Physical chemistry
Impact factor: 11.205, year: 2020
Method of publishing: Open access
https://doi.org/10.1073/pnas.2017379117

Fluorescent proteins have been used extensively in many areas of life sciences. Many of their applications rely on their various biophysical properties, such as excitation and emission wavelengths, excited state lifetimes, or sensitivity to the molecular environment. One aspect of fluorescent proteins that has remained largely neglected is the directionality of their optical properties. In the present work, we describe our experimental determination of the directionality of light absorption and emission in several commonly used fluorescent proteins. Our findings improve our understanding of fundamental properties of fluorescent proteins, and expand the possibilities of development and applications of genetically encoded fluorescent probes.
Permanent Link: http://hdl.handle.net/11104/0316288