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Antitumor and antioxidant activities of purple potato ethanolic extract and its interaction with liposomes, albumin and plasmid DNA
- 1.0537129 - ÚFCH JH 2022 RIV GB eng J - Journal Article
Strugata, P. - Urbaniak, A. - Kuryś, P. - Vloch, A. - Ugorski, M. - Hof, Martin - Gabrielska, J.
Antitumor and antioxidant activities of purple potato ethanolic extract and its interaction with liposomes, albumin and plasmid DNA.
Food & Function. Roč. 12, č. 3 (2021), s. 1271-1290. ISSN 2042-6496. E-ISSN 2042-650X
R&D Projects: GA ČR(CZ) GX19-26854X
Institutional support: RVO:61388955
Keywords : antitumor * antioxidant activities * DNA
OECD category: Physical chemistry
Impact factor: 6.317, year: 2021
Method of publishing: Limited access
The aim of study was to broadly determine the biological activities of purple potato ethanolic extract of the Blue Congo variety (BCE). The antioxidative activity of BCE was determined in relation to liposome membranes, and peroxidation was induced by UVB and AAPH. To clarify the antioxidant activity of BCE, we investigated its interactions with hydrophilic and hydrophobic regions of a membrane using fluorimetric and FTIR methods. Next, we investigated the cytotoxicity and pro-apoptotic activities of BCE on two human colon cancer cell lines (HT-29 and Caco-2) and on normal cells (IPEC-J2 ). In addition, the ability to inhibit enzymes which are involved in pro-inflammatory reactions was examined. Furthermore, BCE interactions with serum albumin and plasmid DNA were investigated using steady state fluorescence spectroscopy and single molecule fluorescence technique (TCSPC-FCS). We proved that BCE effectively protects lipid membranes against the process of peroxidation and successfully inhibits the cyclooxygenase and lipoxygenase enzymes. What is more, it interacts with the hydrophilic and hydrophobic parts of lipid membranes as well as with albumin and plasmid DNA. It occurred, that BCE is more cytotoxic against colon cancer cell lines than normal IPEC-J2 cells, it also induces apoptosis in cancer cell lines, but does not induce cell death on normal cells.
Permanent Link: http://hdl.handle.net/11104/0314875
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