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Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium
- 1.0537030 - BC 2021 RIV CH eng J - Journal Article
Pernicová, I. - Nováčková, I. - Sedláček, P. - Kouřilová, X. - Kalina, M. - Kovalčik, A. - Koller, M. - Nebesářová, Jana - Krzyžánek, Vladislav - Hrubanová, Kamila - Másílko, J. - Slaninova, E. - Obruča, S.
Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium.
Polymers. Roč. 12, č. 6 (2020), č. článku 1235. E-ISSN 2073-4360
R&D Projects: GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_013/0001775
Institutional support: RVO:60077344 ; RVO:68081731
Keywords : waste glycerol * poly(3-hydroxybutyrate) * bacillus * biosynthesis * poly(3-hydroxybutyrate-co-3-hydroxyvalerate) * polyhydroxyalkanoates * thermophiles * Aneurinibacillussp * P(3HB-co-4HB) * P(3HB-co-3HV-co-4HB)
OECD category: Genetics and heredity (medical genetics to be 3); Electrical and electronic engineering (UPT-D)
Impact factor: 4.329, year: 2020
Method of publishing: Open access
https://www.mdpi.com/2073-4360/12/6/1235
Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolateAneurinibacillussp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 degrees C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated thatAneurinibacillussp. H1 is a very promising candidate for production of PHA with tailored material properties.
Permanent Link: http://hdl.handle.net/11104/0314787
Number of the records: 1