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Separation of the glycosylated carotenoid myxoxanthophyll from synechocystis salina by hpccc and evaluation of its antioxidant, tyrosinase inhibitory and immune-stimulating properties

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    0536963 - MBÚ 2021 RIV CH eng J - Journal Article
    Nováková, Michaela - Fábryová, Tereza - Vokurková, D. - Dolečková, I. - Kopecký, Jiří - Hrouzek, Pavel - Tůmová, L. - Cheel, José
    Separation of the glycosylated carotenoid myxoxanthophyll from synechocystis salina by hpccc and evaluation of its antioxidant, tyrosinase inhibitory and immune-stimulating properties.
    Separations. Roč. 7, č. 4 (2020), č. článku 73. E-ISSN 2297-8739
    R&D Projects: GA MŠk(CZ) LO1416
    Institutional support: RVO:61388971
    Keywords : Centrifugal partition chromatography (CPC) * Countercurrent chromatography (CCC) * Cyanobacteria * High-performance countercurrent chromatography (HPCCC) * Myxoxanthophyll
    Subject RIV: EE - Microbiology, Virology
    OECD category: Microbiology
    Impact factor: 2.777, year: 2020
    Method of publishing: Open access
    https://www.mdpi.com/2297-8739/7/4/73

    Global demand for natural pigments has increased in the past few years. Myxoxanthophyll, a glycosylated monocyclic carotenoid, is a pigment that occurs naturally in cyanobacteria but no scalable isolation process has been developed to obtain it from its natural source to date. In this study, myxoxanthophyll was isolated from unicellular cyanobacterium Synechocystis salina (S. salina) using high-performance countercurrent chromatography (HPCCC), where the lower phase of the biphasic solvent system composed of n-heptane–ethanol–water (2:4:4, v/v/v) was used as a mobile phase, whereas its upper phase was the stationary phase. For the HPCCC isolation, a multi-injection method was developed, and four consecutive sample injections (70 mg each) were performed, obtaining, in total, 20 mg of myxoxanthophyll, which was finally purified with high-performance liquid chromatography (HPLC). Overall, a final myxoxanthophyll yield of 15 mg (98% purity) was obtained. The target pigment showed a weak antioxidant and tyrosinase inhibitory effect, and exhibited immune-stimulating properties by activating human granulocytes. The results presented here form a basis for the large-scale production of myxoxanthophyll, and show the potential benefits of this pigment for human health.
    Permanent Link: http://hdl.handle.net/11104/0314711

     
     
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