Translational Research in Serious Human Diseases

0536511 - ÚŽFG 2021 RIV CZ eng M - Monography Chapter
Červenka, Jakub
System wide proteomic approaches - methods and applications in neurodegeneration research.
Translational Research in Serious Human Diseases. Praha: Academia, 2020 - (Kello, M.; Strnadel, J.; Klempir, J.; Roth, J.; Myslivcová-Fučíková, A.; Hansíková, H.; Kozák, I.), s. 92-101. ISBN 978-80-200-3158-7
R&D Projects: GA MŠMT(CZ) LO1609; GA MŠMT(CZ) LTC18079
Institutional support: RVO:67985904
Keywords : proteomics * antibody microarray * flow cytometry
OECD category: Biochemistry and molecular biology

Omics approaches became popular in last decades due to their undeniable asset in providing complex information about system of interest, e.g. in vitro cultivated cell population. Their main benefit is a large number of analytes (e.g. RNAs or proteins) monitored in parallel. As proteins are responsible for phenotype, proteomics is the technique of choice. Two main diverse proteomic approaches exist – antibody-based or mass spectrometry-based approach. This review is focused on characterisation of different methods from both approaches, their descriptions and examples of their applications in research of neurodegenerative diseases.
Permanent Link: http://hdl.handle.net/11104/0314287