Large volume sample stacking of antiepileptic drugs in counter current electrophoresis performed in PAMAPTAC coated capillary

0536465 - ÚOCHB 2022 RIV NL eng J - Journal Article
Tůma, P. - Hložek, T. - Sommerová, B. - Koval, Dušan
Large volume sample stacking of antiepileptic drugs in counter current electrophoresis performed in PAMAPTAC coated capillary.
Talanta. Roč. 221, Jan 1 (2021), č. článku 121626. ISSN 0039-9140. E-ISSN 1873-3573
Institutional support: RVO:61388963
Keywords : antiepileptic * capillary electrophoresis * coating * contactless conductivity detection * sample pretreatment * serum * stacking
OECD category: Analytical chemistry
Impact factor: 6.556, year: 2021
Method of publishing: Limited access
https://doi.org/10.1016/j.talanta.2020.121626

Electrophoretic stacking is developed for sensitive determination of three zwitterionic antiepileptics, namely vigabatrin, pregabalin and gabapentin, in human serum. CE separation is performed in a 25 μm fused silica capillary covalently coated with the copolymer of acrylamide with 5% content of permanently charged 3-acrylamidopropyl trimethylammonium chloride (PAMAPTAC). In background electrolyte of 500 mM acetic acid, the 5% PAMAPTAC generates an anodic electro-osmotic flow with a magnitude of (−18.6 ± 0.5) · 10−9 m2V−1s−1, which acts against the direction of the electrophoretic migration of the analytes. A sample of the antiepileptic prepared in a 25% v/v infusion solution and 75% v/v acetonitrile is injected into the capillary in a large volume attaining a zone length of up to 270 mm. After turning on the separation voltage, the antiepileptics are isotachophoretically focussed behind the zone of Na+ ions with a sensitivity enhancement factor of 78. For the clinical determination of antiepileptics, the human serum is diluted with acetonitrile in a ratio of 1:3 v/v and a zone with a length of 90 mm is injected into the capillary. The method is linear in the 0.025–2.5 μg/mL concentration range, the attained limit of quantification is in the range 18.3–22.8 nmol/L, the within-day precision for the migration time is 0.8–1.2% and for the peak area 1.5–2.4%.
Permanent Link: http://hdl.handle.net/11104/0316386