In-cell NMR Spectroscopy of Nucleic Acids

0536192 - BFÚ 2021 RIV GB eng J - Journal Article
Dzatko, S. - Fiala, R. - Haensel-Hertsch, R. - Foldynová-Trantírková, Silvie - Trantírek, L.
In-cell NMR Spectroscopy of Nucleic Acids.
New Developments in NMR. New Developments in NMR. - (Ito, Y.; Dotsch, V.; Shirakawa, M.). Roč. 21, č. 2020 (2020), s. 272-297. ISBN 978-1-78801-307-9. ISSN 2044-253X
R&D Projects: GA MŠMT EF15_003/0000477
Institutional support: RVO:68081707
Keywords : g-quadruplex structures * reversible membrane permeabilization * human telomeric dna * living cells * water suppression * molecular switch * gene-expression
OECD category: Biochemistry and molecular biology
Method of publishing: Limited access
https://pubs.rsc.org/en/content/chapter/bk9781788012171-00272/978-1-78801-217-1

The structure, dynamics, and interactions of nucleic acids, in particular DNAs, sensitively respond to a broad range of environmental factors, many of them being natively present in the intracellular space. In-cell NMR is a tailored adaptation of conventional high-resolution nuclear magnetic resonance spectroscopy that permits characterization of stereochemical features of nucleic acids directly in living cells. Here we review recent advances and applications of in-cell NMR to nucleic acids. The limitations of this method as well as the general considerations, comprising of critical factors to be accounted for during the planning of in-cell NMR experiments, as well as during the interpretation of in-cell NMR data, are discussed.
Permanent Link: http://hdl.handle.net/11104/0314002