Functional analysis of adipokinetic hormone signaling in Bombyx mori

0536147 - BC 2021 RIV CH eng J - Journal Article
Sehadová, Hana - Takasu, Y. - Žaloudíková, Anna - Lin, Yu-Hsien - Šauman, Ivo - Sezutsu, H. - Rouhová, Lenka - Kodrík, Dalibor - Žurovec, Michal
Functional analysis of adipokinetic hormone signaling in Bombyx mori.
Cells. Roč. 9, č. 12 (2020), č. článku 2667. E-ISSN 2073-4409
Institutional support: RVO:60077344
Keywords : silkworm * targeted mutagenesis * TALEN
OECD category: Biochemistry and molecular biology
Impact factor: 6.600, year: 2020
Method of publishing: Open access
https://www.mdpi.com/2073-4409/9/12/2667/pdf

Insect adipokinetic hormones (AKHs) are short peptides produced in the corpora cardiaca and are responsible for mobilizing energy stores from the fat body to the hemolymph. Three related peptides, AKH1, AKH2, and AKH/corazonin-related peptide (ACP) as well as three AKH receptors have been reported in Bombyx mori. AKH1 and AKH2 are specific for the AKHR1 receptor, whereas ACP interacts with the other two AKHRs. To assess the e ect of the two silkworm AKHs and ACP in the regulation of energy homeostasis we examined the expression pattern of the three peptides and their receptors as well as their e ect on the level of carbohydrates and lipids in the hemolymph. Our results support the hypothesis that only AKH1 and AKH2 peptides together with the AKHR1 receptor are involved in the maintenance of energy homeostasis. Because Bombyx AKHR1 (BmAKHR1) seems to be a true AKHR we generated its mutation. The BmAKHR1 mutant larvae display significantly lower carbohydrate and lipid levels in the hemolymph and reduced sensitivity to starvation. Our study clarifies the role of BmAKHR1 in energy homeostasis.
Permanent Link: http://hdl.handle.net/11104/0316033