Substitution-Inert Polynuclear Platinum Complexes Inhibit Reverse Transcription Preferentially in RNA Triplex-Forming Templates

0536128 - BFÚ 2021 RIV US eng J - Journal Article
Malina, Jaroslav - Farrell, N.P. - Brabec, Viktor
Substitution-Inert Polynuclear Platinum Complexes Inhibit Reverse Transcription Preferentially in RNA Triplex-Forming Templates.
Inorganic Chemistry. Roč. 59, č. 20 (2020), s. 15135-15143. ISSN 0020-1669. E-ISSN 1520-510X
R&D Projects: GA ČR(CZ) GC20-14082J
Institutional support: RVO:68081707
Keywords : RNA * Triplex-Forming * Substitution-Inert Polynuclear Platinum Complexes
OECD category: Inorganic and nuclear chemistry
Impact factor: 5.165, year: 2020
Method of publishing: Limited access
https://pubs.acs.org/doi/10.1021/acs.inorgchem.0c02070

RNA triplexes are significant tertiary structure motifs that
are found in many functional RNAs. Hence, small molecules capable of recognition, binding, and stabilization of the triple-helical RNA structures are emerging as attractive potential molecular biology tools and therapeutic agents. Here, we utilize methods of molecular biology and biophysics to study the interactions of a series of antitumor substitution-inert polynuclear platinum complexes (SI-PPCs) with triple-helical RNA structures. We show that SI-PPCs recognize and stabilize RNA triplexes and inhibit reverse transcription preferentially in the RNA template prone to the triplex formation. These so far unexplored properties of SI-PPCs suggest that the targeting of triplestranded regions in RNA might contribute to the biological effects of SIPPCs.
Permanent Link: http://hdl.handle.net/11104/0313952