Effect of diamond-like carbon doped with chromium on cell differentiation, immune activation and apoptosis

0535844 - FGÚ 2021 RIV CH eng J - Journal Article
Trávníčková, Martina - Vandrovcová, Marta - Filová, Elena - Steinerová, Marie - Racková, Jana - Kocourek, Tomáš - Bartová, J. - Suchý, Tomáš - Žaloudková, Margit - Jelínek, Miroslav - Bačáková, Lucie
Effect of diamond-like carbon doped with chromium on cell differentiation, immune activation and apoptosis.
European Cells and Materials. Roč. 40, Nov 30 (2020), s. 276-302. ISSN 1473-2262. E-ISSN 1473-2262
R&D Projects: GA ČR(CZ) GA15-05864S; GA ČR(CZ) GA20-01570S; GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:67985823 ; RVO:68378271 ; RVO:67985891
Keywords : chromium * biocompatibility * mesenchymal stromal cells * apoptosis * osteogenic differentiation * macrophages
OECD category: Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction); Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction) (USMH-B)
Impact factor: 3.942, year: 2020
Method of publishing: Open access
https://www.ecmjournal.org/papers/vol040/pdf/v040a17.pdf

Diamond-like carbon (DLC) is a biocompatible material that has many potential biomedical applications, including in orthopaedics. DLC layers doped with Cr at atomic percent (at.%) of 0, 0.9, 1.8, 7.3, and 7.7 at.% were evaluated with reference to their osteoinductivity with human bone marrow mesenchymal stromal cells (hMSCs), immune activation potential with RAW 264.7 macrophage-like cells, and their effect on apoptosis in Saos-2 human osteoblast-like cells and neonatal human dermal fibroblasts (NHDFs). At mRNA level, hMSCs on DLC doped with 0.9 and 7.7 at.% of Cr reached higher maximum values of both RUNX2 and alkaline phosphatase. An earlier onset of mRNA production of type I collagen and osteocalcin was also observed on these samples, they also supported the production of both type I collagen and osteocalcin. RAW 264.7 macrophages were screened using a RayBio™ Human Cytokine Array for cytokine production. 10 cytokines were at a concentration more than 2 × as high as the concentration of a positive control, but the values for the DLC samples were only moderately higher than the values on glass. NHDF cells, but not Saos-2 cells, had a higher expression of pro-apoptotic markers Bax and Bim and a lower expression of anti-apoptotic factor BCL-XL in proportion to the Cr content. Increased apoptosis was also proven by annexin V staining. These results show that a Cr-doped DLC layer with a lower Cr content can act as an osteoinductive material with relatively low immunogenicity, but that a higher Cr content can induce cell apoptosis.
Permanent Link: http://hdl.handle.net/11104/0314531