Identification of polycomb repressive complex 1 and 2 core components in hexaploid bread wheat

0534960 - ÚEB 2021 RIV GB eng J - Journal Article
Strejčková, Beáta - Čegan, Radim - Pečinka, Aleš - Milec, Zbyněk - Šafář, Jan
Identification of polycomb repressive complex 1 and 2 core components in hexaploid bread wheat.
BMC Plant Biology. Roč. 20, OCT 14 (2020), č. článku 175. ISSN 1471-2229. E-ISSN 1471-2229
R&D Projects: GA ČR(CZ) GA19-05445S; GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : Epigenetics * Histone methylation * Polycomb repressive complex * prc2 * Wheat
OECD category: Biochemistry and molecular biology
Impact factor: 4.215, year: 2020
Method of publishing: Open access
http://doi.org/10.1186/s12870-020-02384-6

Background: Polycomb repressive complexes 1 and 2 play important roles in epigenetic gene regulation by posttranslationally modifying specific histone residues. Polycomb repressive complex 2 is responsible for the trimethylation of lysine 27 on histone H3, Polycomb repressive complex 1 catalyzes the monoubiquitination of histone H2A at lysine 119. Both complexes have been thoroughly studied in Arabidopsis, but the evolution of polycomb group gene families in monocots, particularly those with complex allopolyploid origins, is unknown. Results: Here, we present the in silico identification of the Polycomb repressive complex 1 and 2 (PRC2, PRC1) subunits in allohexaploid bread wheat, the reconstruction of their evolutionary history and a transcriptional analysis over a series of 33 developmental stages. We identified four main subunits of PRC2 [E(z), Su(z), FIE and MSI] and three main subunits of PRC1 (Pc, Psc and Sce) and determined their chromosomal locations. We found that most of the genes coding for subunit proteins are present as paralogs in bread wheat. Using bread wheat RNA-seq data from different tissues and developmental stages throughout plant ontogenesis revealed variable transcriptional activity for individual paralogs. Phylogenetic analysis showed a high level of protein conservation among temperate cereals. Conclusions: The identification and chromosomal location of the Polycomb repressive complex 1 and 2 core components in bread wheat may enable a deeper understanding of developmental processes, including vernalization, in commonly grown winter wheat.
Permanent Link: http://hdl.handle.net/11104/0313089