Ribosome-Mediated Attenuation of vga(A) Expression Is Shaped by the Antibiotic Resistance Specificity of Vga(A) Protein Variants

0534627 - MBÚ 2021 RIV US eng J - Journal Article
Vimberg, Vladimír - Cavanagh, J. P. - Novotná, Michaela - Lenart, Jakub - Nguyen Thi Ngoc, Bich - Veselá, Jana - Pain, M. - Koběrská, Markéta - Balíková Novotná, Gabriela
Ribosome-Mediated Attenuation of vga(A) Expression Is Shaped by the Antibiotic Resistance Specificity of Vga(A) Protein Variants.
Antimicrobial Agents and Chemotherapy. Roč. 64, č. 11 (2020), č. článku e00666-20. ISSN 0066-4804. E-ISSN 1098-6596
R&D Projects: GA ČR GJ15-16225Y; GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61388971
Keywords : staphylococcus-aureus st398 * soft-tissue infections * streptogramin * peptide * pleuromutilins * lincosamides * haemolyticus * ABCF proteins * Staphylococcus haemolyticus * Vga(A) * antibiotic resistance * clindamycin
OECD category: Microbiology
Impact factor: 5.191, year: 2020
Method of publishing: Limited access
https://aac.asm.org/content/64/11/e00666-20

Vga(A) protein variants confer different levels of resistance to lincosamides, streptogramin A, and pleuromutilins (LSAP) by displacing antibiotics from the ribosome. Here, we show that expression of vga(A) variants from Staphylococcus haemolyticus is regulated by cis-regulatory RNA in response to the LSAP antibiotics by the mechanism of ribosome-mediated attenuation. The specificity of induction depends on Vga(A)-mediated resistance rather than on the sequence of the riboregulator. Fine tuning between Vga(A) activity and its expression in response to the antibiotics may contribute to the selection of more potent Vga(A) variants because newly acquired mutation can be immediately phenotypically manifested.
Permanent Link: http://hdl.handle.net/11104/0312852