Development of a 3D bone model for biomedical research

0534606 - ÚIACH 2021 DE eng A - Abstract
Killinger, Michael - Švandová, Eva - Kratochvílová, Adéla - Veselá, Barbora - Matalová, Eva - Klepárník, Karel
Development of a 3D bone model for biomedical research.
Organoids: Modelling Organ Development and Disease in 3D Culture. Heidelberg: EMBL Heidelberg, 2020. s. 133-133.
[EMBO | EMBL Symposium: Organoids: Modelling Organ Development and Disease in 3D Culture. 21.10.2020-24.10.2020, Virtual Conference]
R&D Projects: GA ČR(CZ) GA20-00726S
Institutional support: RVO:68081715
Keywords : bone * 3D * biotechnology
OECD category: Analytical chemistry

Three-dimensional (3D) culture systems known as organoids derived from precursor cells are accessible for many tissues with a limited number of studies available for bone. The system allows the appropriate cell-cell interactions, however, achieving 3D spheroids from
the bone-related cells is still rare. Here we established a simple method for culture of 3D bone spheroids that can be used for bone research, translation medicine, disease modelling and regeneration. We also designed the prototype of a miniaturized culture chambre inside a Petri dish provided by continual perfusion of nutrients to minimalize a necrotic core formation and support osteogenic differentiation. The system also includes a live imaging system to observe the behaviour of cells.
Permanent Link: http://hdl.handle.net/11104/0312778