Progressive lysosomal membrane permeabilization induced by iron oxide nanoparticles drives hepatic cell autophagy and apoptosis

0534437 - FZÚ 2021 RIV US eng J - Journal Article
Levada, K. - Pshenichnikov, S. - Omelyanchik, A. - Rodionova, V. - Nikitin, A. - Savchenko, A. - Schetinin, I. - Zhukov, D. - Abakumov, M. - Majouga, A. - Lunova, Mariia - Jirsa, M. - Smolková, Barbora - Uzhytchak, Mariia - Dejneka, Alexandr - Lunov, Oleg
Progressive lysosomal membrane permeabilization induced by iron oxide nanoparticles drives hepatic cell autophagy and apoptosis.
Nano Convergence. Roč. 7, č. 1 (2020), s. 1-17, č. článku 17. ISSN 2196-5404. E-ISSN 2196-5404
R&D Projects: GA MŠMT LTC19040
Institutional support: RVO:68378271
Keywords : cytotoxicity * apoptosis * autophagy * iron oxide nanoparticles * magnetic resonance imaging
OECD category: Biophysics
Impact factor: 8.526, year: 2020
Method of publishing: Open access
https://doi.org/10.1186/s40580-020-00228-5

Iron oxide nanoparticles (IONs) are frequently used in various biomedical applications, in particular as magnetic resonance imaging contrast agents in liver imaging. Indeed, number of IONs have been withdrawn due to their poor clinical performance. Yet comprehensive understanding of their interactions with hepatocytes remains relatively limited. Here we investigated how iron oxide nanocubes (IO-cubes) and clusters of nanocubes (IO-clusters) affect distinct human hepatic cell lines. The viability of HepG2, Huh7 and Alexander cells was concentration-dependently decreased after exposure to either IO-cubes or IO-clusters. We found similar cytotoxicity levels in three cell lines triggered by both nanoparticle formulations. Our data indicate that different expression levels of Bcl-2 predispose cell death signaling mediated by nanoparticles.
Permanent Link: http://hdl.handle.net/11104/0312631