Iron oxide nanoparticle-induced autophagic flux is regulated by interplay between p53-mTOR axis and Bcl-2 signaling in hepatic cells

0534433 - FZÚ 2021 RIV CH eng J - Journal Article
Uzhytchak, Mariia - Smolková, Barbora - Lunova, Mariia - Jirsa, M. - Frtús, Adam - Kubinová, Šárka - Dejneka, Alexandr - Lunov, Oleg
Iron oxide nanoparticle-induced autophagic flux is regulated by interplay between p53-mTOR axis and Bcl-2 signaling in hepatic cells.
Cells. Roč. 9, č. 4 (2020), s. 1-24, č. článku 1015. E-ISSN 2073-4409
R&D Projects: GA MŠMT LTC19040
Institutional support: RVO:68378271
Keywords : nano-bio interactions * iron oxide nanoparticles * autophagy * lysosomes * magnetic resonance imaging * p53
OECD category: Biophysics
Impact factor: 6.600, year: 2020
Method of publishing: Open access

Iron oxide-based nanoparticles have been repeatedly shown to affect lysosomal-mediated signaling. Recently, nanoparticles have demonstrated an ability to modulate autophagic flux via lysosome-dependent signaling. However, the precise underlying mechanisms of such modulation as well as the impact of cellular genetic background remain enigmatic. In this study, we investigated how lysosomal-mediated signaling is a ected by iron oxide nanoparticle uptake in three distinct hepatic cell lines. We found that nanoparticle-induced lysosomal dysfunction alters sub-cellular localization of pmTOR and p53 proteins. Our data indicate that alterations in the sub-cellular localization of p53 protein induced by nanoparticle greatly affect the autophagic flux.

Permanent Link: http://hdl.handle.net/11104/0312628