Light-induced modulation of the mitochondrial respiratory chain activity: possibilities and limitations

0534420 - FZÚ 2021 RIV CH eng J - Journal Article
Lunova, Mariia - Smolková, Barbora - Uzhytchak, Mariia - Janoušková, K.Ž. - Jirsa, M. - Egorova, D. - Kulikov, A. - Kubinová, Šárka - Dejneka, Alexandr - Lunov, Oleg
Light-induced modulation of the mitochondrial respiratory chain activity: possibilities and limitations.
Cellular and Molecular Life Sciences. Roč. 77, č. 14 (2020), s. 2815-2838. ISSN 1420-682X. E-ISSN 1420-9071
R&D Projects: GA MŠMT(CZ) EF16_019/0000760
Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Institutional support: RVO:68378271
Keywords : phototoxicity * apoptosis * Reactive oxygen species (ROS) * high fluence low-power laser irradiation * hhotobiomodulation * hitochondrial membrane potential (Delta m phi)
OECD category: Biophysics
Impact factor: 9.261, year: 2020
Method of publishing: Limited access
https://doi.org/10.1007/s00018-019-03321-z

Biological effects of high fluence low-power (HFLP) lasers have been reported for some time, yet the molecular mechanisms procuring cellular responses remain obscure. A better understanding of the effects of HFLP lasers on living cells will be instrumental for the development of new experimental and therapeutic strategies. Therefore, we investigated sub-cellular mechanisms involved in the laser interaction with human hepatic cell lines. We show that mitochondria serve as sub-cellular “sensor” and “effector” of laser light non-specific interactions with cells. We demonstrated that despite blue and red laser irradiation results in similar apoptotic death, cellular signaling and kinetic of biochemical responses are distinct. Our findings reveal the mechanism how HFLP laser irradiation interfere with cell homeostasis and underscore that such laser irradiation permits remote control of mitochondrial function in the absence of chemical or biological agents.
Permanent Link: http://hdl.handle.net/11104/0312619