Phytochemical composition and in vitro biological activity of iris spp. (iridaceae): A new source of bioactive constituents for the inhibition of oral bacterial biofilms

0533874 - BÚ 2021 RIV CH eng J - Journal Article
Hoang, L. - Beneš, F. - Fenclová, M. - Kronusová, O. - Švarcová, V. - Řehořová, K. - Baldassarre Švecová, Eva - Vosátka, Miroslav - Hajšlová, J. - Kaštánek, P. - Viktorová, J. - Ruml, T.
Phytochemical composition and in vitro biological activity of iris spp. (iridaceae): A new source of bioactive constituents for the inhibition of oral bacterial biofilms.
Antibiotics (Basel). Roč. 9, č. 7 (2020), s. 1-20, č. článku 403. ISSN 2079-6382. E-ISSN 2079-6382
Institutional support: RVO:67985939
Keywords : biofilm * dental plaque * quorum sensing * microbial resistance
OECD category: Organic chemistry
Impact factor: 4.639, year: 2020
Method of publishing: Open access
https://doi.org/10.3390/antibiotics9070403

The publication reported on the chemical composition and the antibiofilm activity of 15 methanolic extracts of Iris species against both mono-(Pseudomonas aeruginosa, Staphylococcus aureus) and multi-species oral biofilms (Streptococcus gordonii, Veillonella parvula, Fusobacterium nucleatum subsp. nucleatum, and Actinomyces naeslundii). The phytochemical profiles of Iris pallida s.l., Iris versicolor L., Iris lactea Pall., Iris carthaliniae Fomin, and Iris germanica were determined by ultra-high performance liquid chromatography-high-resolution tandem mass spectroscopy (UHPLC-HRMS/MS) analysis, and a total of 180 compounds were identified among Iris species with (iso)flavonoid dominancy. I. pallida, I. versicolor, and I. germanica inhibited both the quorum sensing and adhesion during biofilm formation in a concentration-dependent manner. However, the extracts were less active against maturated biofilms. Of the five tested species, Iris pallida s.l. was the most effective at both inhibiting biofilm formation and disrupting existing biofilms, and the leaf extract exhibited the strongest inhibitory effect compared to the root and rhizome extracts. The cytotoxicity of the extracts was excluded in human fibroblasts. The inhibition of bacterial adhesion significantly correlated with myristic acid content, and quorum sensing inhibition correlated with the 7-β-hydroxystigmast-4-en-3-one content. These findings could be useful for establishing an effective tool for the control of oral biofilms and thus dental diseases.
Permanent Link: http://hdl.handle.net/11104/0313703