Rapid Isolation, Propagation, and Online Analysis of a Small Number of Therapeutic Staphylococcal Bacteriophages from a Complex Matrix

0533184 - ÚIACH 2021 RIV US eng J - Journal Article
Horká, Marie - Šalplachta, Jiří - Karásek, Pavel - Růžička, F. - Štveráková, D. - Pantůček, R. - Roth, Michal
Rapid Isolation, Propagation, and Online Analysis of a Small Number of Therapeutic Staphylococcal Bacteriophages from a Complex Matrix.
ACS Infectious Diseases. Roč. 6, č. 10 (2020), s. 2745-2755. ISSN 2373-8227
R&D Projects: GA ČR(CZ) GA19-00742S; GA MZd(CZ) NV16-29916A; GA MV VI20172020069
Institutional support: RVO:68081715
Keywords : electormigration techniques and MALDI-TOF MS * nano-etched FS capillary * bacteriophage
OECD category: Analytical chemistry
Impact factor: 5.084, year: 2020
Method of publishing: Limited access

A method for the fast isolation, propagation, and characterization of very low count bacteriophages from the maximum 10 mL blood plasma sample active against pathogenic bacterial strains was developed. Bacteriophages with a count of 100 phage particles were dynamically adhered onto the nanostructured part of the fused silica capillary.
Permanent Link: http://hdl.handle.net/11104/0311654