Role of Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Metabolic Adaptations of Prostate Cancer

0532976 - FGÚ 2021 RIV CH eng J - Journal Article
Pecinová, Alena - Alán, Lukáš - Brázdová, Andrea - Vrbacký, Marek - Pecina, Petr - Drahota, Zdeněk - Houštěk, Josef - Mráček, Tomáš
Role of Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Metabolic Adaptations of Prostate Cancer.
Cells. Roč. 9, č. 8 (2020), č. článku 1764. E-ISSN 2073-4409
R&D Projects: GA ČR(CZ) GA16-12726S; GA MŠMT(CZ) ED1.1.00/02.0109
Grant - others:AV ČR(CZ) StrategieAV21/7
Program: StrategieAV
Institutional support: RVO:67985823
Keywords : GPD2 gene * mitochondrial glycerol-3-phosphate dehydrogenase (EC:1.1.5.3) * prostate cancer * metabolic adaptation
OECD category: Biochemistry and molecular biology
Impact factor: 6.600, year: 2020
Method of publishing: Open access
https://www.mdpi.com/2073-4409/9/8/1764

Prostate cancer is one of the most prominent cancers diagnosed in males. Contrasting with other cancer types, glucose utilization is not increased in prostate carcinoma cells as they employ different metabolic adaptations involving mitochondria as a source of energy and intermediates required for rapid cell growth. In this regard, prostate cancer cells were associated with higher activity of mitochondrial glycerol-3-phosphate dehydrogenase (mGPDH), the key rate limiting component of the glycerophosphate shuttle, which connects mitochondrial and cytosolic processes and plays significant role in cellular bioenergetics. Our research focused on the role of mGPDH biogenesis and regulation in prostate cancer compared to healthy cells. We show that the 42 amino acid presequence is cleaved from N-terminus during mGPDH biogenesis. Only the processed form is part of the mGPDH dimer that is the prominent functional enzyme entity. We demonstrate that mGPDH overexpression enhances the wound healing ability in prostate cancer cells. As mGPDH is at the crossroad of glycolysis, lipogenesis and oxidative metabolism, regulation of its activity by intramitochondrial processing might represent rapid means of cellular metabolic adaptations.
Permanent Link: http://hdl.handle.net/11104/0311482