Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage

0532829 - ÚEB 2021 RIV GB eng J - Journal Article
Montesinos, J. C. - Abuzeineh, A. - Kopf, A. - Juanes-Garcia, A. - Ötvös, K. - Petrášek, Jan - Sixt, M. - Benková, E.
Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage.
EMBO Journal. Roč. 39, č. 17 (2020), č. článku e104238. ISSN 0261-4189. E-ISSN 1460-2075
R&D Projects: GA MŠMT(CZ) EF16_019/0000738
Institutional support: RVO:61389030
Keywords : cell differentiation * cytokinin * cytoskeleton * microtubules * microtubules dynamics
OECD category: Biochemical research methods
Impact factor: 11.598, year: 2020
Method of publishing: Open access
http://doi.org/10.15252/embj.2019104238

Cell production and differentiation for the acquisition of specific functions are key features of living systems. The dynamic network of cellular microtubules provides the necessary platform to accommodate processes associated with the transition of cells through the individual phases of cytogenesis. Here, we show that the plant hormone cytokinin fine-tunes the activity of the microtubular cytoskeleton during cell differentiation and counteracts microtubular rearrangements driven by the hormone auxin. The endogenous upward gradient of cytokinin activity along the longitudinal growth axis in Arabidopsis thaliana roots correlates with robust rearrangements of the microtubule cytoskeleton in epidermal cells progressing from the proliferative to the differentiation stage. Controlled increases in cytokinin activity result in premature re-organization of the microtubule network from transversal to an oblique disposition in cells prior to their differentiation, whereas attenuated hormone perception delays cytoskeleton conversion into a configuration typical for differentiated cells. Intriguingly, cytokinin can interfere with microtubules also in animal cells, such as leukocytes, suggesting that a cytokinin-sensitive control pathway for the microtubular cytoskeleton may be at least partially conserved between plant and animal cells.
Permanent Link: http://hdl.handle.net/11104/0311217