Homozygous transgenic barley (Hordeum vulgare L.) plants by anther culture

0532719 - ÚEB 2021 RIV CH eng J - Journal Article
Ohnoutková, Ludmila - Vlčko, Tomáš
Homozygous transgenic barley (Hordeum vulgare L.) plants by anther culture.
Plants. Roč. 9, č. 7 (2020), s. 1-11, č. článku 918. E-ISSN 2223-7747
R&D Projects: GA MŠMT(CZ) EF16_019/0000738
Institutional support: RVO:61389030
Keywords : Androgenesis barley * Double haploid * Embryo culture * Transformation
OECD category: Biochemical research methods
Impact factor: 3.935, year: 2020
Method of publishing: Open access
http://doi.org/10.3390/plants9070918

Production of homozygous lines derived from transgenic plants is one of the important steps for phenotyping and genotyping transgenic progeny. The selection of homozygous plants is a tedious process that can be significantly shortened by androgenesis, cultivation of anthers, or isolated microspores. Doubled haploid (DH) production achieves complete homozygosity in one generation. We obtained transgenic homozygous DH lines from six different transgenic events by using anther culture. Anthers were isolated from T0 transgenic primary regenerants and cultivated in vitro. The ploidy level was determined in green regenerants. At least half of the 2n green plants were transgenic, and their progeny were shown to carry the transgene. The process of dihaploidization did not affect the expression of the transgene. Embryo cultures were used to reduce the time to seed of the next generation. The application of these methods enables rapid evaluation of transgenic lines for gene function studies and trait evaluation.
Permanent Link: http://hdl.handle.net/11104/0311131