Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa, a wild relative of common wheat

0532642 - ÚEB 2021 RIV CN eng J - Journal Article
Lei, J. - Zhou, J. - Sun, H. - Wan, W. - Xiao, J. - Yuan, C. - Karafiátová, Miroslava - Doležel, Jaroslav - Wang, H. - Wang, X.
Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa, a wild relative of common wheat.
Crop Journal. Roč. 8, č. 4 (2020), s. 676-681. ISSN 2095-5421. E-ISSN 2214-5141
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : Chromosome identification * Haynaldia villosa * Oligonucleotide probes * Tandem DNA repeats
OECD category: Biochemistry and molecular biology
Impact factor: 4.407, year: 2020
Method of publishing: Open access
http://doi.org/10.1016/j.cj.2020.02.008

Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement. Owing to the limited number of probes available for fluorescence in situ hybridization (FISH), the resolution at which the karyotype of H. villosa can be characterized is poor, hampering accurate characterization of small segmental alien introgressions. We designed ten oligonucleotide probes using tandem repeats in DNA sequences derived from the short arm of H. villosa chromosome 6V (6VS). FISH with seven of them resulted in clear signals on H. villosa chromosomes. Using these, we constructed FISH karyotypes for H. villosa using oligo-6VS-1 and oligo-6VS-35 oligonucleotides and characterized the distribution of the two probes in five different H. villosa accessions. The new FISH probes can efficiently characterize H. villosa introgressions into wheat.
Permanent Link: http://hdl.handle.net/11104/0311069