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Photon-upconversion barcode for monitoring an enzymatic reaction with a fluorescence reporter in droplet microfluidics
- 1.0532623 - ÚIACH 2021 RIV GB eng J - Journal Article
Hlaváček, Antonín - Křivánková, Jana - Pizúrová, Naděžda - Václavek, Tomáš - Foret, František
Photon-upconversion barcode for monitoring an enzymatic reaction with a fluorescence reporter in droplet microfluidics.
Analyst. Roč. 145, č. 23 (2020), s. 7718-7723. ISSN 0003-2654. E-ISSN 1364-5528
R&D Projects: GA ČR(CZ) GJ18-03367Y; GA MŠMT(CZ) 8F17003
Institutional support: RVO:68081715 ; RVO:68081723
Keywords : photon-upconversion * droplet microfluidics * fluorescence signal
OECD category: Analytical chemistry; Nano-materials (production and properties) (UFM-A)
Impact factor: 4.616, year: 2020
Method of publishing: Limited access
We report luminescent photon-upconversion barcodes for indexing the chemical content of droplets. The barcode is compatible with the simultaneous detection of fluorescence. The encoding and decoding of the initial concentration of enzyme β-galactosidase and substrate 4-methylumbelliferyl β-D-galactopyranoside are described. The fluorescent product 4-methylumbelliferone is detected simultaneously with the barcode.
Permanent Link: http://hdl.handle.net/11104/0311057
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