Extraordinary Sequence Diversity and Promiscuity of Centromeric Satellites in the Legume Tribe Fabeae.

0532455 - BC 2021 RIV US eng J - Journal Article
Robledillo, Laura Avila - Neumann, Pavel - Koblížková, Andrea - Novák, Petr - Vrbová, Iva - Macas, Jiří
Extraordinary Sequence Diversity and Promiscuity of Centromeric Satellites in the Legume Tribe Fabeae.
Molecular Biology and Evolution. Roč. 37, č. 8 (2020), s. 2341-2356. ISSN 0737-4038. E-ISSN 1537-1719
R&D Projects: GA ČR(CZ) GA17-09750S; GA MŠMT(CZ) LM2015047
Institutional support: RVO:60077344
Keywords : centromere evolution * satellite DNA * CENH3 * ChIP-seq * plant chromosomes
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 16.240, year: 2020
Method of publishing: Open access
https://academic.oup.com/mbe/article/37/8/2341/5817320

Satellite repeats are major sequence constituents of centromeres in many plant and animal species. Within a species, a single family of satellite sequences typically occupies centromeres of all chromosomes and is absent from other parts of the genome. Due to their common origin, sequence similarities exist among the centromere-specific satellites in related species. Here, we report a remarkably different pattern of centromere evolution in the plant tribe Fabeae, which includes genera Pisum, Lathyrus, Vicia, and Lens. By immunoprecipitation of centromeric chromatin with CENH3 antibodies, we identified and characterized a large and diverse set of 64 families of centromeric satellites in 14 species. These families differed in their nucleotide sequence, monomer length (33-2,979bp), and abundance in individual species. Most families were species-specific, and most species possessed multiple (2-12) satellites in their centromeres. Some of the repeats that were shared by several species exhibited promiscuous patterns of centromere association, being located within CENH3 chromatin in some species, but apart from the centromeres in others. Moreover, FISH experiments revealed that the same family could assume centromeric and noncentromeric positions even within a single species. Taken together, these findings suggest that Fabeae centromeres are not shaped by the coevolution of a single centromeric satellite with its interacting CENH3 proteins, as proposed by the centromere drive model. This conclusion is also supported by the absence of pervasive adaptive evolution of CENH3 sequences retrieved from Fabeae species.
Permanent Link: http://hdl.handle.net/11104/0310942