Simultaneous determination of tumour biomarkers homovanillic acid, vanillylmandelic acid, and 5-hydroxyindole-3-acetic acid in human urine using single run HPLC with a simple wall-jet glassy carbon electrochemical detector

0532429 - ÚFCH JH 2021 RIV CH eng J - Journal Article
Němečková-Makrlíková, Anna - Barek, J. - Navrátil, Tomáš - Fischer, J. - Vyskočil, V. - Dejmková, H.
Simultaneous determination of tumour biomarkers homovanillic acid, vanillylmandelic acid, and 5-hydroxyindole-3-acetic acid in human urine using single run HPLC with a simple wall-jet glassy carbon electrochemical detector.
Journal of Electroanalytical Chemistry. Roč. 878, DEC 2020 (2020), č. článku 114629. ISSN 1572-6657. E-ISSN 1873-2569
R&D Projects: GA ČR(CZ) GA20-01589S
Institutional support: RVO:61388955
Keywords : homovanillic acid * Vanillylmandelic acid * 5-Hydroxyindole-3-acetic acid
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Impact factor: 4.464, year: 2020
Method of publishing: Limited access

HPLC with amperometric detection (HPLC-ED) was used for the simultaneous determination of tumour biomarkers homovanillic acid (HVA), vanillylmandelic acid (VMA), and 5-hydroxyindole-3-acetic acid (5-HIAA) in one run in human urine using inexpensive lab-made glassy carbon wall-jet detector. Simple and fast solid phase extraction was used instead of earlier used more time consuming liquid-liquid extraction. Simultaneous determination of the three analytes in one run is fast with limits of detection at micromolar concentrations. Amperometric detection is more sensitive and selective than the spectrophotometric one. Calibration dependences are linear in the concentration range from 20 to 150 μmol·L−1. Interferences of other compounds present in urine were not observed for HPLC-ED. Concentrations of tumour biomarkers in urine of healthy volunteer found by HPLC-ED correspond with previously published normal physiological concentrations.
Permanent Link: http://hdl.handle.net/11104/0310923