Interaction Between Zn Deficiency, Toxicity and Turnip Yellow Mosaic Virus Infection in Noccaea ochroleucum

0532417 - BC 2021 RIV CH eng J - Journal Article
Morina, Filis - Mishra, Archana - Mijovilovich, Ana - Matoušková, Šárka - Brueckner, D. - Špak, Josef - Küpper, Hendrik
Interaction Between Zn Deficiency, Toxicity and Turnip Yellow Mosaic Virus Infection in Noccaea ochroleucum.
Frontiers in Plant Science. Roč. 11, JUN 5 (2020), č. článku 739. ISSN 1664-462X. E-ISSN 1664-462X
R&D Projects: GA MŠMT EF15_003/0000336
Institutional support: RVO:60077344 ; RVO:67985831
Keywords : cadmium * chlorophyll fluorescence kinetics * metal transporters * non-hyperaccumulator * plant immunity * TYMV infection * zinc * micro X-ray fluorescence
OECD category: Biochemistry and molecular biology; Biochemistry and molecular biology (GLU-S)
Impact factor: 5.754, year: 2020
Method of publishing: Open access
https://www.frontiersin.org/articles/10.3389/fpls.2020.00739/full

Zinc is essential for the functioning of numerous proteins in plants. To investigate how Zn homeostasis interacts with virus infection, Zn-tolerantNoccaea ochroleucumplants exposed to deficient (Zn'0'), optimal (Zn10), and excess Zn (Zn100) concentrations, as well as Cd amendment, were infected with Turnip yellow mosaic virus (TYMV). Imaging analysis of fluorescence kinetics from the mu s (OJIP) to the minutes (Kautsky effect, quenching analysis) time domain revealed strong patchiness of systemic virus-induced photosystem II (PSII) inhibition. That was more pronounced in Zn-deficient plants, while Zn excess acted synergistically with TYMV, in both cases resulting in reduced PSII reaction centers. Infected Cd-treated plants, already severely stressed, showed inhibited non-photochemical quenching and PSII activity. Quantitativein situhybridization at the cellular level showed increased gene expression ofZNT5and downregulation ofHMA4in infected Zn-deficient leaves. In Zn10 and Zn100 infected leaves, vacuolar sequestration of Zn increased by activation ofHMA3(mesophyll) andMTP1(epidermis). This correlated with Zn accumulation in the mesophyll and formation of biomineralization dots in the cell wall (Zn100) visible by micro X-ray fluorescence tomography. The study reveals the importance of adequate Zn supply and distribution in the maintenance of photosynthesis under TYMV infection, achieved by tissue-targeted activation of metal transporter gene expression.
Permanent Link: http://hdl.handle.net/11104/0310913