Remdesivir triphosphate can efficiently inhibit the RNA-dependent RNA polymerase from various flaviviruses

0532046 - ÚOCHB 2021 RIV NL eng J - Journal Article
Konkoľová, Eva - Dejmek, Milan - Hřebabecký, Hubert - Šála, Michal - Böserle, Jiří - Nencka, Radim - Bouřa, Evžen
Remdesivir triphosphate can efficiently inhibit the RNA-dependent RNA polymerase from various flaviviruses.
Antiviral Research. Roč. 182, Oct (2020), č. článku 104899. ISSN 0166-3542. E-ISSN 1872-9096
R&D Projects: GA MZd(CZ) NU20-05-00472; GA MŠMT(CZ) EF16_019/0000729
Institutional support: RVO:61388963
Keywords : remdesivir * inhibitor * flavivirus * RNA-dependent RNA polymerase
OECD category: Organic chemistry
Impact factor: 5.970, year: 2020
Method of publishing: Limited access
https://doi.org/10.1016/j.antiviral.2020.104899

Remdesivir was shown to inhibit RNA-dependent RNA-polymerases (RdRp) from distinct viral families such as from Filoviridae (Ebola) and Coronaviridae (SARS-CoV, SARS-CoV-2, MERS). In this study, we tested the ability of remdesivir to inhibit RdRps from the Flaviviridae family. Instead of remdesivir, we used the active species that is produced in cells from remdesivir, the appropriate triphosphate, which could be directly tested in vitro using recombinant flaviviral polymerases. Our results show that remdesivir can efficiently inhibit RdRps from viruses causing severe illnesses such as Yellow fever, West Nile fever, Japanese and Tick-borne encephalitis, Zika and Dengue. Taken together, this study demonstrates that remdesivir or its derivatives have the potential to become a broad-spectrum antiviral agent effective against many RNA viruses.
Permanent Link: http://hdl.handle.net/11104/0310659