Fluorinated water-soluble poly(2-oxazoline)s as highly sensitive 19F MRI contrast agents

0531986 - ÚMCH 2021 RIV US eng J - Journal Article
Sedlacek, O. - Jirák, D. - Vít, M. - Ziolkowska, N. - Janoušková, Olga - Hoogenboom, R.
Fluorinated water-soluble poly(2-oxazoline)s as highly sensitive 19F MRI contrast agents.
Macromolecules. Roč. 53, č. 15 (2020), s. 6387-6395. ISSN 0024-9297. E-ISSN 1520-5835
R&D Projects: GA MŠMT(CZ) LO1507
Institutional support: RVO:61389013
Keywords : imaging agents * magnetic resonance imaging * fluorine
OECD category: Polymer science
Impact factor: 5.985, year: 2020
Method of publishing: Limited access
https://pubs.acs.org/doi/10.1021/acs.macromol.0c01228

Recently, 19F magnetic resonance imaging (MRI) emerged as a powerful noninvasive diagnostic tool in modern medicine. Fluorinated polymer materials represent an attractive class of MRI contrast agents (CAs) due to their structural variability and tunable properties. Herein, we describe for the first time the 19F MRI of CAs based on fluorinated water-soluble poly(2-oxazoline)s (PAOx), a polymer class with increasing popularity in biomedical sciences. A series of fluorinated PAOx with increasing fluorine content were synthesized by controlled side-chain hydrolysis of poly(2-methyl-2-oxazoline) followed by reacylation of its ethylenimine units by difluoroacetic anhydride. As the increasing fluorine content leads to the copolymer hydrophobization, their composition was optimized for maximal 19F MRI performance while retaining good solubility in water. The magnetic properties of the water-soluble polymers were studied in vitro by 19F NMR and MRI, revealing their outstanding relaxation properties and imaging sensitivity. All CAs were found to be noncytotoxic for HeLa cells in vitro. Finally, the diagnostic potential of the new CAs was demonstrated by a successful in vivo19F MRI visualization of the selected fluorinated polymer in rats.
Permanent Link: http://hdl.handle.net/11104/0310684